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Nb35 was purified by size-exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 75 column (GE Healthcare) with running buffer containing 20 mM HEPES, 100 mM NaCl, pH 7.4

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Lean meats include sirloin, tenderloin, and lean ground beef

Embark cited data from IWSR (Ciobanu, 2024), a leading source on drinks trends, which reported a decline in sales in all alcohol categories last year, including wine and champagne

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