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and a 500 mcg level, a higher potency that can be leveraged as a loading level or applied to cases where more support is needed

That is rate-dependent and expected

Any of these is grounds for discarding the vial

Instead, fundoscopy demonstrated only discrete generalized vessel caliber irregularity with mild atrophy of the optic disc, and then, quite rapidly, normal eye background and choroidal blood vessels, which remain in all of the subsequent periods