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As anticipated, RSL3 treatment suppressed mTOR signaling pathway in PTC cells in vitro

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The RT2 mix was added to the RT1 mixture (final volume: 25 L), and the reaction was carried out in a thermal cycler: 90 min at 37 C, followed by 5 min at 95 C, and held at 4 C

Subsequent examination of membranous GLUT1 expression in both tissues revealed that TXNIP overexpression led to a significant decrease of GLUT1 expression in the ileum and colon after metformin treatment, where metformin increased GLUT1 expression (Fig

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