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however, in combination with 0.01 M MTX was significantly able to reduce cell viability

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ROS are highly reactive, unstable molecules our bodies produce during normal metabolism and in response to environmental stresses

The tissues were fixed in 4% paraformaldehyde and then embedded in paraffin

Subsequently the tissues were processed as for immunofluorescence

Although de novo polyamine synthesis from ornithine represents the major source of intracellular polyamines, transport of extracellular polyamines into cells is accomplished via the SLC45A4 encoded transporter which is a member of the glycoside-pentoside-hexuronide cation symporter family of transporters