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Freezing a reconstituted peptide solution can cause: Ice crystal formation that mechanically disrupts peptide structure Phase separation or precipitation upon thawing Reduced bioactivity in subsequent experiments Refrigerator storage maintains the peptide in a stable aqueous environment

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Images were acquired 24 48 h later, on a Keyence BZ-X800 microscope using negative control sections to adjust for background fluorescence

In addition, both peptides form multiple hydrophobic interactions with the TM1 residues of GLP-1R