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Vitamin B6 is required for serine hydroxymethyltransferase (SHMT) function in the folate cycle and also in the conversion of homocysteine to cysteine in the transsulfuration pathway

10.1073/pnas.1500624112 Proc

Methylcobalamin is the pre-activated form that bypasses hepatic conversion and does not trigger the same mast cell degranulation response, making it the preferred option for histamine-sensitive individuals

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Freeze-thaw: Avoid repeated freeze-thaw cycles of reconstituted solution

Affinity purified protein samples were further resolved by size exclusion chromatography and assessed by SDS-PAGE and negative stain TEM before vitrification and cryo-EM imaging (Supplementary Fig