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For the non-active state, particle subtraction was performed for both ATD-focused and LBD-TMD-focused heterogeneous refinement

A., Beduneau, A., Pellequer, Y

& Korac, B

Non-targeted lipidomic analysis revealed that levels of lysophosphatidylcholines (LysoPCs) and lysophosphatidylethanolamines (LysoPEs) were significantly increased in both GCH1-expressing and overexpressing cells following IKE treatment and contained PUFAs

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In total, 10 l washed beads were then added to each resuspended pellet and shaken at room temperature for 2 h to form the biotinstreptavidin interaction