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Primers and qRT-PCR Pomc were AAGAGGCTAGAGGTCATCAG and AGAACGCCATCATCAAGAAC, for OPRM1 CAGGGCTTGTCCTTGTAAGAAA and GACTCGGTAGGCTGTAACTGA, for OPRD ATGGCCTCATGCTACTGCG and CACCAGCGTCCAGACGATG, for OPRK TCCCCAACTGGGCAGAATC and GACAGCGGTGATGATAACAGG, for 18S AGGTTCTGGCCAACGGTCTAG and CCCTCTATGGGCTCGAATTTT, for Actb GGCTGTATTCCCCTCCATCG and CCAGTTGGTAACAATGCCATGT, and for Hprt1 TCAGTCAACGGGGGACATAAA and GGGGCTGTACTGCTTAACCAG

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As experienced facilitators, we find ways of using by-products from one partner as raw material for another