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On days two and three, significantly higher MIC values between the 5 day Zn pre-exposure condition and the zero day zinc pre exposure condition were observed, with the 5 day Zn pre-exposure condition displaying a MIC value nearly three times as high compared to the MIC of the zero day Zn-pre-exposure condition (Figure 1B, day two: p = 0.0061, day three: p = 0.0176 as analyzed in GraphPad Prism via ordinary one-way ANOVA with multiple comparisons via Dunnets test with a sample size of N = 4 and error bars representing the standard deviation)

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The AUC for plasma GLP-1 concentration was calculated using the conventional trapezoidal method

Caution applies for individuals with cardiovascular conditions, though topical application minimizes systemic exposure