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Moreover, NAMPT activity is crucial for the beneficial effects of calorie restriction, including improved oxidative stress management, mitochondrial biogenesis, and metabolic adaptation, as reported in a study involving Sprague-Dawley rats 213

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Samples were allowed to cool, and 1.75 ml purified water with a resistivity 18.2 M cm from a Milli-Q system (Merck Millipore) was added to make up to a final volume of 2 ml