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When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature

Not all sites on the body provide equal access to this layer or equal comfort during injection

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While a single case has limitations, it demonstrates the extract's rapid anti-inflammatory potential

GHK-Cu 7-30 /

GHK-Cu has the larger skin and wound-healing evidence base