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Several factors can affect results: Age and sex Pregnancy Fasting status Hydration Recent exercise Acute illness Supplements and other medications Anemia Kidney function Weight and body-composition changes Laboratory methodology An abnormal result does not always indicate disease, and a normal result does not always exclude a clinically important problem

10.1210/en.2006-0153 [DOI] [PubMed] [Google Scholar] van Dijk, G

As an important guard against experimenter effects biasing results, participants, investigators, and outcome assessors were all not told participants experimental condition (i.e., they were kept blind to study condition)

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Eur J Clin Invest 54(12):e14292 Virani SS, Newby LK, Arnold SV, Bittner V, Brewer LC, Demeter SH et al (2023) 2023 AHA/ACC/ACCP/ASPC/NLA/PCNA Guideline for the management of patients with chronic coronary disease

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