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The plotter will generate a curve showing drug accumulation over the first 4 to 5 weeks until steady state is achieved

From a toxicology standpoint, a pleiotropic, multi-pathway mechanism is a double-edged observation: it may explain the broad tissue tolerability seen so far, but it also widens the surface area of biology that a complete safety program would need to interrogate, because a molecule that touches many pathways has many pathways through which an unintended long-term effect could emerge

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The pelleted cells were washed twice with 0.85% NaCl, resuspended in 1 mL 30% methanol/water, and lysed on ice with ultrasonication

Kardiologiia 60 , 104109 (2021)