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Primary structure properties of fusion proteins Physical and chemical characteristics of engineered fusion proteins, including all molecular weight, molecular formula, theoretical isoelectric point (pI), the total number of positive and negatively charged residues in the sequence, extinction coefficient (E), instability index (II), aliphatic index (AI), and grand average hydropathy (GRAVY), were attained using the Expasy ProtParam server 63

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doi: 10.2967/jnumed.116.187492 119 BashiriFAHudairiAHamadMHAl-SulimaniLKAl HomyaniDAl SaqabiDet al

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The day before the experiment, 2,000 cells/well were seeded in 96-well plates (Corning)

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