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Rapid loss of body fat can sometimes cause muscle atrophy (loss of lean muscle) and a decrease in skin elasticity, leading to sagging

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J Clin Lipidol 2016;10:1081-90.ArticlePubMed 669

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Absorbance at 410 nm was measured once every minute at 7 time points using Microplate Spectrophotometer PowerWave XS(BioTek Instruments, Winooski, VT, USA)

Gawaz, M., Dickfeld, T., Bogner, C., Fateh-Moghadam, S