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A volume of 2 l samples was injected into a Waters XBridge XP BEH Amide column (150 mm length 2.1 mm i.d., 2.5 m particle size) maintained at 25 C, with a Waters XBridge XP VanGuard BEH Amide (5 mm 2.1 mm i.d., 2.5 m particle size) guard column

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53.4A) back to the active metabolite

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