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Nat Rev Cancer 2:311315

Bawa JA, Mohammed I, Muhammad SG, et al

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From the compound-target-disease target network, 235 potential targets were identified

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MCF-7 Cas9-expressing cells were transduced in independent triplicate samples of 1.73 10 8 cells each with the lentiviral whole-genome sgRNA library at a multiplicity of infection of 0.25-0.29, as measured by puromycin resistance, to achieve a representation of 252287 cells per sgRNA