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Apamin structure and pharmacology revisited

When NF-kB translocates to the cell nucleus, it switches on the transcription of pro-inflammatory cytokines including TNF-alpha, IL-6, and IL-1-beta

GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating

Sometimes we don't need these high force interventions

PMID: 31686879

Both agents demonstrate favorable effects on cardiovascular risk factors, including blood pressure reduction and lipid profile improvement