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[10] Scratch assays: These tests measure how quickly a gap in a cell layer closes

Heal leaky gut and rebalance your microbiome

A subsequent study of the same region reported increased TRP levels and an elevated KYN/TRP ratio, with KP activation extending to the downstream metabolite 3-HAA [234]

Adding GIP (the tirzepatide model) deepens the effect and, somewhat counterintuitively, appears to improve gastrointestinal tolerability relative to GLP-1 alone