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4570-C REEVALUATING THE TREATMENT INDICATIONS FOR PRIMARY BILIARY CHOLANGITIS - ANALYSIS OF A NATIONWIDE SURVEY COHORT IN JAPAN- Kosuke Matsumoto 1 , Junko Hirohara 2 , Toshiaki Nakano 2 and Atsushi Tanaka 1 , (1)Teikyo University School of Medicine, (2)Kansai Medical University Background: PBC Clinical practice guidelines state that patients with abnormal ALP values are eligible for treatment, but there is no clear evidence supporting this recommendation

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Ionomycin was used as a positive control

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Wells were then washed with PBS and collected by trypsinization