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doi: 10.1016/j.peptides.2019.04.004 133 TomabechiYKrippnerGRendlePMSquireMAFairbanksAJ

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PCR reactions were carried out using 0.5 L of gene-specific cDNA samples and that of 16S rRNA as templates with 1 mM final concentration of each of the forward and reverse RT primers (Additional file 12: Table S5) and 4 L of 2 SYBR green PCR mix in a final volume of 10 L