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In the future, longitudinal studies will be necessary to capture trajectories of changes over time in AC levels and depressive symptoms in order to properly disentangle trait vs state effects and provide empirical grounding for causal interpretation, triangulating evidence with experimental medicine approaches

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The rAAV-Ef1-DIO-mCherry-WPRE-pA (AAV-DIO-mCherry, AAV2/9, 2.12 10 12 vg/mL) virus was used as the control

That part is going to be up to you, and this article was written to help you navigate these murky waters with ease