For BODIPY staining, fixed cells were stained by a BODIPY solution (1 g/ml), and representative images were captured under a fluorescence microscope
have demonstrated that PPAR expression is downregulated in autosomal dominant polycystic kidney disease (ADPKD) and in primary cells from human ADPKD patients [296, 297]
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Examples of research applications include: Cellular redox investigations Oxidative metabolism research Biochemical signalling studies Antioxidant pathway analysis Comparative molecular investigations Intracellular biochemical modelling Controlled in vitro biochemical studies These applications are intended exclusively for scientific and laboratory investigation
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With respect to insulin receptor signaling proteins, insulin receptor substrate-1 (IRS-1), phosphatidylinositol-3-kinase (PI3K), protein kinase B/AK strain transforming (AKT/PKB), PIP 3 -dependent kinase 1 (PDPK1), and glycogen synthase kinase 3 (GSK3) are all known to be O -GlcNAcylated
