Membranes were sedimented by ultracentrifugation at 150,000 g at 4 C for 1 h, and resuspended in 16 mL of solubilized buffer (1%(w/v) DDM, 150 mM NaCl, 10%(v/v) glycerol, 1 mg mL 1 iodoacetamide, 0.2 mM TCEP, 0.1 protease inhibitor cocktail, 50 mM Tris-HCl pH 8.0)
doi: 10.3390/nu11102278
Symptoms of vitamin D toxicity are due to increased calcium levels in the blood and can include nausea, vomiting, muscle weakness, neurologic or psychiatric symptoms, pain, loss of appetite, dehydration, increased urination, excessive thirst, and kidney stones
As TriNetX is a global dataset we also utilized TriNetX Curated (TNX Curated) codes, which are similar terms combined to minimize redundancy of database coding, Anatomical Therapeutic Chemical (ATC), RxNorm, and ICD-9 coding systems
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They ultimately did have liposuction where these symptoms did improve