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The skin around eyes is thinner and more sensitive

doi:10.1208/s12249-020-01872-0 106

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Afterwards, the cells were centrifuged (20,000 g, 10 min), washed with 1 PBS, re-counted, and adjusted to 4 10 5 yeast cells/mL in the respective medium

In old age 3xTg-AD neurons, bound GTP remained localized at the periphery