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Moreover, arsenic trioxide, nitrosourea, e.g., busulfan or carmustine, or natural compounds, such as curcumine(-derivates) or piperlongumine, are potent inhibitors of the Trx and Grx systems via inhibition of TrxR, GR, or depletion of GSH [207,208] and inducers of ferroptosis [208-211]

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The cells were then scraped on ice, transferred to pre-chilled microcentrifuge tubes, centrifuged at 15,000 g for 20 min and the supernatants were collected

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