Fisher, Targeting the (Un)differentiated state of Cancer
Together, excessive and reduced production of neuro-toxic and -protective kynurenines, respectively, may exacerbate excitotoxicity and neuroinflammation, establishing a feed-forward cycle of KP dysregulation, 5-HT depletion, HPA axis hyperactivity, and increased vulnerability to MDD
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But remember the 28-day stability rule: once reconstituted, the solution should be used within 28 days
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Cells were incubated at 37 C with 5% CO 2 overnight to attach to plates, rinsed in PBS, then placed in starved media overnight before treatment