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At Mansoura Universitys Physiology Department, Faculty of Veterinary Medicine, the rats were kept in separate plastic cages, at the rate of six per cage, with wood shavings as bedding

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Proper evaluation and clinical supervision are essential to reduce risks and ensure suitability for each individual

For Evotip loading, any remaining beads were removed by plate-based filtration (Merck, 10448023), and half the filtered digest volume was loaded on Evotips according to the manufacturers instructions

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