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Push the needle in fully

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Cells were then washed and stained with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen, 1:1000) for 30 min

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Part of the spinal cord gray and white matter was used for analysis under light microscopy (magnification 300)

Thus, GPX1 overexpressing -cells produce more insulin, whereas -cell mass is reduced in GPx1 deficient mice, leading to hypoinsulinemia and decreased glucose-stimulated insulin secretion [96]