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Patients with certain FTO patterns experience stronger nausea from dietary fat and benefit from plant-based proteins, while others tolerate moderate fat intake without side effects

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Similarly to Sox2 , the promoter methylation of transient fate determinants of the bHLH and SoxC families was also not detected neither in stem nor in differentiated cells (Fouse et al., 2008

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Step 3: Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37C