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The cleavage of GST from the target protein can be challenging, especially if the cleavage site is not efficiently recognized by the protease or if the GST tag affects the accessibility of the cleavage site

Eur J Biochem 67: 231238

This decrease from the expected fluorescent conjugate might be due to quenching of the fluorescence by excessive amount of MBB (greater than 50 M) surrounding the conjugate

The same study also showed that AtGSTU1 and AtGTU24 genes were significantly altered and involved in susceptibility

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