2-10 nmol of L-glutamate standards and the cell lysate were mixed with reaction buffer (final concentrations of 36.8 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH 7.1), 2.1910-5 U L-glutamate oxidase, 1 U peroxidase, 0.8 mM 3.5-dimethoxy-N-ethyl-N-(2-hydroxy-3-sulphopropyl) aniline (sodium salt) (DAOS), 0.8 mM aminoantipyrine)
Together these findings, therefore, suggest that alsin protects cells from oxidative stress and could be a redox sensing protein, similar to Rab5, Rac1, and SOD1
Lee JS, Pan J-J, Ramamoorthy G, Poulter CD (2017) Structurefunction studies of artemisia tridentata farnesyl diphosphate synthase and chrysanthemyl diphosphate synthase by site-directed mutagenesis and morphogenesis
Biomolecules 8, 170
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In one trial, 136 patients with non-small cell lung cancer were randomized to receive chemotherapy alone (paclitaxel and carboplatin) or in combination with high-dose antioxidant supplements
