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Possible reactions may include digestive discomfort, headaches, skin reactions, or rare allergic responses

10.3389/fimmu.2018.02823 102 QiY.YamauchiY.LingJ.KawanoN.LiD.TanakaK

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The tissue was then incubated in Hanks buffered salt solution (HBSS) containing 15 mM HEPES, 50 U/mL collagenase type II (Thermo Fisher 17101015), and 100 U/mL hyaluronidase (Sigma Aldrich H3506) at 37 C for 30 min

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Its an apt name, as they are essential for life, however they are also nutrients that the body cannot synthesize on its own