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In brief, male mice were deeply anaesthetized with an intraperitoneal injection of 7% chloral hydrate and transcardially perfused with a modified ice-cold artificial cerebrospinal fluid (aCSF) (described below)

The peptide appeared to induce activation of ribosomal genes and decondensation of the heterochromatin

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While hGH affects glucose tolerance and insulin sensitivity, AOD9604 showed no adverse effects

Children with ASD commonly exhibit increased intestinal permeabilityoften referred to as leaky gutwhich allows microbial metabolites, such as short-chain fatty acids (SCFAs) and neuroactive compounds (e.g., 5-HT, GABA), to enter systemic circulation (Ullah et al., 2024)