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However, these florescent probes are susceptible to various drawbacks such as photo-bleaching and molecular leaching from the host, which may result in serious experimental artifacts 6,7,8,9

Acknowledgements This work was supported by the Science and Technology Innovation Committee of Shenzhen Municipality (No

doi:10.1074/jbc.M601876200

Without a concentration label, you risk dosing errors if you mix multiple peptides or use different reconstitution volumes across vials

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