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Not optional, not recommended, but required for maintaining the 28-day beyond-use date and preventing bacterial colonization

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PMID 25738459 ScienceDirect / Cell Metabolism Commentary on Lee et al

doi: 10.1016/j.jad.2011.06.005

A2 An amount of 450 mL of the suspension (step A1) was added to 450 mL of DMEM and 10 g of matrix/100 mL sterile hydroalcoholic solution (90% H 2 0/10% EtOH) (total volume 1 L

Metabolic reprogramming is required for both malignant transformation and tumor development, including invasion and metastasis