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Determination of Nitric Oxide (NO) Production The nitrite (NO 2 ) was estimated colorimetrically at 12 and 24 h post CLP on the basis of reduction of nitrate to nitrite using Griess method ( Bacterial Load Determination For determination of colony-forming units (CFU) in blood and peritoneal fluid of mice ( n = 6 per/group), 10 L of samples were diluted with sterile Phosphate-Buffered Saline (PBS) 1:10, and then 10 L of each dilution was cultured in Mller Hinton Agar and incubated at 37 C for 24 h

Moreover, there are credible evidences that GEN produced hepatoprotection through modulating the expression and phosphorylation of epidermal growth factor receptor (EGFR) in experimental fibrosis (Rodriguez and Alvarez, 2016)

Oncotarget, 6(19), 17832

Available at: (Accessed Sepember 11, 2017)

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