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Extending to Other Qualifying variants, 61/80 (76%) of participants presented with at least one Qualifying variant in these genes

Samples were then evaporated to dryness in Speed vac and resuspended in 500 l Buffer A (95% H 2 O, 5% CH 3 CN, 0.1% FA) with sonication and loaded on HPLC for offline high pH fractionation following conditions adapted from previous work 57

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Understanding these distinctions is essential for anyone serious about long-term metabolic health

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