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Before adding 5 L of plasma in a 96 well plate, a reaction mixture containing 5 U/mL of Lactate dehydrogenase (LDH-A) enzyme dissolved in 0.1 M phosphate buffer (pH 6.5), 200 M NAD + and 50 M Hydrazine Hydrate was prepared

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These macromolecules include DNA, proteins, and lipids (74)

Therefore, the relationship needs to be further verified in clinical samples

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years)

Nygrd et al, 1997a)