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First, we expressed and purified APTF-2::V5-containing nuclear fractions from mammalian HEK293T cells

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Bioinformatic predictions on subcellular protein localization were obtained from DeepLoc-1.0 ( and predictions on transmembrane domains and/or signal peptides from Phobius ( Analyses on nucleotide and protein sequences were done with Clustal Omega ( provided by the EMBL's European Bioinformatics Institute (Heidelberg, Germany) or the Swiss Institute of Bioinformatics using the Expasy Translate tool ( Arabidopsis mutants Double-mutant plants lacking cytosolic G6PDH activity were kindly provided by Claudia Jonak (Vienna, Austria)

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