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HEK-GLP-1R cells or wild-type HEK-293 cells were grown in ELISA 8-well strips (96-well format) and used either with or without loading with the Ca2+ indicator, fluo-4 as indicated

Caught late (after months or years), some damage may not fully recover

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The first and rate-limiting step, catalyzed by glutamate-cysteine ligase (GCL), generates -glutamylcysteine from glutamate and cysteine

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doi: 10.1016/S0006-8993(00)02741-4 224 BomfimTRForny-GermanoLSathlerLBBrito-MoreiraJHouzelJCDeckerHet al