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The nutritionist identified suboptimal protein intake based on the patients dietary recall form

RIPA Cell Lysis Buffer 2 (Enzo Life Sciences, Farmingdale, NY), Protease Inhibitor Cocktail, Phenylmethylsulfonyl fluoride (PMSF) at 1 mM concentration, and 20 g/mL concentration of DNase (Sigma Aldrich, St Louis, MO)

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repeat for two to three minutes to relax the diaphragm