For real-time TEER, Caco-2/HT29-MTX cells (90:10) were seeded at a concentration of 4.8 10 5 cells/well (2 mL/well) in a 6-well plate containing polyester permeable-membrane inserts (basolateral chamber = 3 mL)
On testing cadence, note that there is no universal evidence-based schedule: the frequency of laboratory monitoring should be individualized according to the therapy being used, underlying medical conditions, treatment response, and current clinical guidelines
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Robert Ashley, MD, is an internist and assistant professor of medicine at the University of California, Los Angeles
Blood pressure regulation through the nitric oxide signaling pathway represents a systemic vascular effect, with hypotensive activity observed in some animal experiments at higher doses
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