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Validation experiment based on parallel reaction monitoring (PRM) To further validate our proteomics data, we selected proteins related to lipid synthesis (Cd36, Fabp2, Fabp4, Plin2, Plin4, and Plin5), fatty acid oxidation (FAO) (Cpt1a, Acaa1b, and Cyp4a10), the mitophagy-lysosome pathway (Bnip3, Sqstm1, Optn, Map1lc3b, Lamp1, Lamp2, and Atp6v0d1), cholesterol metabolism (Abdg5, Abcg8, Cyp7a1, and Hnf4a), and the oxidative stress response (Gstm2, Hmox1, and Hspb1) for targeted proteomics analysis
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BPC-157 focuses on tendon, ligament, and GI tissue through angiogenesis pathways
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