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Other factors, including tobacco smoke, pollution, unbalanced diet, genetic inefficiencies, and use of prescription and/or over the counter drugs (most notably acetaminophen) can further compound this loss. From Centre dEnseignement et de Recherche en Nutrition (CERNh)

This makes every single variable in your experimental setupespecially dosageabsolutely critical

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After 5 days of incubation at 37 C, 100 rpm, microaerophilic conditions, 5% CO 2 , 10% O 2 and 85% N 2, the FoR was calculated as the mean number of mutants divided by the mean number of colonies detected on the growth control