glutathione trapping assay Schematic chemical reaction of the Total (adapted Dansyl Glutathione as a Trapping

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15, 16 Primary antibodies to mouse K14 (ab7800, 1:500), p63 (ab53039, 1:100), PCNA (ab29, 1:200) and cleaved Caspase3 (ab13847, 1:100) were purchased from Abcam

GH triggers neuroendocrine adaptations during weight loss AgRP neurons express c-Fos during food deprivation as an indicator of increased cell activity 14, 15

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In addition, glutathione participates at the ascorbate-glutathione pathway which plays role at ROS detoxifying (Maslennikova and Shakirova 2021)

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