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The difference between total glutathione as AccQ-2-mercaptoethanol double conjugate and reduced-form glutathione in the intestines and liver was insignificant, indicating that most of the food-derived glutathione in the intestine and liver were in the reduced form (Figs

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150 L of 0.2 mol/L pH 7.8 phosphate buffer, 50 L of proxy matrix, and 10 L of 0.001 mol/L N-Hcy-Lys were mixed with 5 L of a mixture, prepared by mixing 0.2 mol/L BCPB solution and 0.25 mol/L TCEP solution in volume ratio 1:1