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Flora, 2009)

Don't leave the vial sitting open or the needle inserted for longer than necessary

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Five hundred l of culture supernatants obtained after centrifugation for 10 min at 5000 g were mixed with an equal volume of PBS containing 1.12 mM phenol red (Sigma) and 19 U horseradish peroxidase (Sigma)

10.3748/wjg.v20.i27.8807 World J

The samples were incubated at 55 C for 30 min to enable reverse transcription, followed by heating at 94 C for 15 min to activate the Hot StartTaq DNA Polymerase