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Safety, effectiveness, specificity, and quality control are important factors for further peptide design

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A phase I clinical trial of the anti-CD28 Ab TGN1412 exemplifies this, as the Ab induced cytokine storms in treated individuals, causing severe damage to the lungs and kidneys, and an immediate need of transfer to the intensive care unit [365]

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The resulting cDNA was used as a template for quantitative RT-PCR, which was performed with primers specific for TGF-1 and -actin (see in Table 1 ), according to the instructions for the SYBR Premix EX Taq Kit (TaKaRa Biotechnology Co., Ltd.), and a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, United States)

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