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These effects are more likely early in a protocol and often ease as the body adapts

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The combination eliminates the need for two separate injections, reduces total injection volume, and ensures consistent dosing ratios optimized for synergistic effects

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Finally, the tissue sections were washed thoroughly with PBS, counterstained with DAPI and imaged under a laser scanning confocal microscope (FV-1000, Olympus, Japan)

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